Formation of an Intermediate in Prostaglandin Biosynthesis and Its Association with the Platelet Release Reaction

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Abstract

A compound that could be converted to prostaglandin F2α by mild chemical reduction was formed by human platelets in response to arachidonic acid, collagen, or L-epinephrine. It was present in maximal amounts at about 1 min after addition of arachidonic acid or collagen to platelet-rich plasma. Its initial formation appeared to precede platelet aggregation by these agents and was closely correlated with the release of adenine nucleotides and radioactive 5-hydroxytryptamine from platelets. Moreover, the compound was itself found outside the platelets. This compound is probably an endoperoxide intermediate in prostaglandin biosynthesis and may be a trigger for the platelet release reaction.

Authors

J. B. Smith, Carol Ingerman, J. J. Kocsis, M. J. Silver