Measurement of δ-Aminolevulinic Acid Synthetase Activity in Human Erythroblasts

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Abstract

A new, specific, and simple method for the determination of δ-aminolevulinic acid (ALA) synthetase activity in human bone marrow cells has been developed. ALA synthetase of erythroblasts was partially purified so as to permit the use of [14C]succinyl-CoA as a substrate for this enzyme. In this enzyme preparation there were negligible activities of succinyl-CoA hydrolase, α-ketoglutarate dehydrogenase, and succinyl-CoA synthetase and there was no activity of ALA dehydrase.

Authors

Yosuke Aoki, Gumpei Urata, Osamu Wada, Fumimaro Takaku