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Isolation of a Novel Glycoprotein from the Urine of a Patient with Chronic Myelocytic Leukemia
Daniel Rudman, … , Alejandro E. Del Rio, Bettye Hollins
Daniel Rudman, … , Alejandro E. Del Rio, Bettye Hollins
Published March 1, 1974
Citation Information: J Clin Invest. 1974;53(3):868-874. https://doi.org/10.1172/JCI107627.
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Research Article

Isolation of a Novel Glycoprotein from the Urine of a Patient with Chronic Myelocytic Leukemia

Abstract

Patient B. J. with chronic myelocytic leukemia excreted 0.5-1.1 g protein per day in the urine. Gel filtration on Sephadex G-75 showed about one-third of this protein to be in molecular weight range 20,000-40,000 (fraction BJC). BJC, prepared from 9 liters of urine by gel filtration, was chromatographed on carboxymethylcellulose. Two proteins were eluted from the resin in pure form (as shown by zone and immunoelectrophoresis) in yields representing 8 and 3 mg/liter of urine: BJC1 and BJC2. Their amino acid compositions were identical. BJC1 contained 61% carbohydrate (33% hexose, 11% sialic acid, 13% glucosamine, 5% galactosamine). BJC2 contained one-fourth to one-half as much of each carbohydrate. Molecular weight of BJC1 was estimated at 29,000 by gel filtration. Neither glycoprotein reacted with rabbit antiserum to normal human serum.

Authors

Daniel Rudman, Rajender K. Chawla, Alejandro E. Del Rio, Bettye Hollins

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