Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article (118)

Advertisement

Free access | 10.1172/JCI107615

Limited Binding Capacity Sites for l-Triiodothyronine in Rat Liver Nuclei: NUCLEAR-CYTOPLASMIC INTERRELATION, BINDING CONSTANTS, AND CROSS-REACTIVITY WITH l-THYROXINE

Jack H. Oppenheimer, Harold L. Schwartz, Diona Koerner, and Martin I. Surks

Endocrine Research Laboratory, Montefiore Hospital, Bronx, New York 10467

Division of Endocrinology, Department of Medicine, Montefiore Hospital, Bronx, New York 10467

Medical Center and the Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10467

Find articles by Oppenheimer, J. in: JCI | PubMed | Google Scholar

Endocrine Research Laboratory, Montefiore Hospital, Bronx, New York 10467

Division of Endocrinology, Department of Medicine, Montefiore Hospital, Bronx, New York 10467

Medical Center and the Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10467

Find articles by Schwartz, H. in: JCI | PubMed | Google Scholar

Endocrine Research Laboratory, Montefiore Hospital, Bronx, New York 10467

Division of Endocrinology, Department of Medicine, Montefiore Hospital, Bronx, New York 10467

Medical Center and the Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10467

Find articles by Koerner, D. in: JCI | PubMed | Google Scholar

Endocrine Research Laboratory, Montefiore Hospital, Bronx, New York 10467

Division of Endocrinology, Department of Medicine, Montefiore Hospital, Bronx, New York 10467

Medical Center and the Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10467

Find articles by Surks, M. in: JCI | PubMed | Google Scholar

Published March 1, 1974 - More info

Published in Volume 53, Issue 3 on March 1, 1974
J Clin Invest. 1974;53(3):768–777. https://doi.org/10.1172/JCI107615.
© 1974 The American Society for Clinical Investigation
Published March 1, 1974 - Version history
View PDF
Abstract

Further studies have been performed to define the kinetic characteristics of nuclear triiodothyronine (T3) binding sites in rat liver (J. Clin. Endocrinol. Metab. 1972. 35: 330). Sequential determination of labeled T3 associated with nuclei and cytoplasm over a 4-h period allowed analysis of the relationship of T3 in nuclear and cytoplasmic compartments. A rapid interchange of hormone between nuclei and cytoplasm was demonstrated, and in vitro incubation experiments with nuclei yielded no evidence favoring metabolic transformation of T3 by the nuclei. In vivo displacement experiments were performed by subcellular fractionation of liver ½ h after injection of [125I]T3 with increasing quantities of unlabeled T3. The nuclear binding capacity for T3 could be defined (0.52 ng/mg DNA). Analysis of these experiments also allowed an estimation of the association constant of nuclear sites for T3 (4.7 × 1011M−1). The affinity of these sites for T3 was estimated to be 20-40 fold greater than for thyroxine (T4). Chromatographic analysis of the nuclear radioactivity after injection of labeled T4 indicated that the binding of T4 by the nucleus could not be attributed to in vivo conversion of T4 to T3 but reflected intrinsic cross-reactivity of the two iodothyronines at the nuclear binding sites.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 768
page 768
icon of scanned page 769
page 769
icon of scanned page 770
page 770
icon of scanned page 771
page 771
icon of scanned page 772
page 772
icon of scanned page 773
page 773
icon of scanned page 774
page 774
icon of scanned page 775
page 775
icon of scanned page 776
page 776
icon of scanned page 777
page 777
Version history
  • Version 1 (March 1, 1974): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article (118)

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts