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Adaption to Hyperoxia: INFLUENCE ON PROTEIN SYNTHESIS BY LUNG AND ON GRANULAR PNEUMOCYTE ULTRASTRUCTURE
Gloria D. Massaro, Donald Massaro
Gloria D. Massaro, Donald Massaro
Published March 1, 1974
Citation Information: J Clin Invest. 1974;53(3):705-709. https://doi.org/10.1172/JCI107608.
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Adaption to Hyperoxia: INFLUENCE ON PROTEIN SYNTHESIS BY LUNG AND ON GRANULAR PNEUMOCYTE ULTRASTRUCTURE

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Abstract

We studied the influence of prolonged exposure to hyperoxia (O2 > 98%) on protein synthesis and on the ultrastructure of the granular pneumocyte. To study protein synthesis, as indicated by l-[U-14C]-leucine incorporation into protein, lung slices were incubated with radioactive leucine and a surface-active fraction was obtained by ultracentrifugation of lung homogenates. We found that, following an initial depression in protein synthesis after 48 h of hyperoxia, protein synthesis in rats exposed to oxygen for 96 h rose to greater than control levels. This increase in protein synthesis was noted in whole lung protein and in protein present in the surface-active fraction.

Authors

Gloria D. Massaro, Donald Massaro

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