Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI107542

Separation of Specific Antibody-Forming Mouse Cells by their Adherence to Insolubilized Endogenous Hormones

Kenneth L. Melmon, Yacob Weinstein, G. M. Shearer, Henry R. Bourne, and Sara Bauminger

Division of Clinical Pharmacology, Department of Pharmacology, University of California Medical Center, San Francisco, California 94143

Division of Clinical Pharmacology, Department of Medicine, University of California Medical Center, San Francisco, California 94143

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel

Find articles by Melmon, K. in: PubMed | Google Scholar

Division of Clinical Pharmacology, Department of Pharmacology, University of California Medical Center, San Francisco, California 94143

Division of Clinical Pharmacology, Department of Medicine, University of California Medical Center, San Francisco, California 94143

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel

Find articles by Weinstein, Y. in: PubMed | Google Scholar

Division of Clinical Pharmacology, Department of Pharmacology, University of California Medical Center, San Francisco, California 94143

Division of Clinical Pharmacology, Department of Medicine, University of California Medical Center, San Francisco, California 94143

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel

Find articles by Shearer, G. in: PubMed | Google Scholar

Division of Clinical Pharmacology, Department of Pharmacology, University of California Medical Center, San Francisco, California 94143

Division of Clinical Pharmacology, Department of Medicine, University of California Medical Center, San Francisco, California 94143

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel

Find articles by Bourne, H. in: PubMed | Google Scholar

Division of Clinical Pharmacology, Department of Pharmacology, University of California Medical Center, San Francisco, California 94143

Division of Clinical Pharmacology, Department of Medicine, University of California Medical Center, San Francisco, California 94143

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel

Find articles by Bauminger, S. in: PubMed | Google Scholar

Published January 1, 1974 - More info

Published in Volume 53, Issue 1 on January 1, 1974
J Clin Invest. 1974;53(1):22–30. https://doi.org/10.1172/JCI107542.
© 1974 The American Society for Clinical Investigation
Published January 1, 1974 - Version history
View PDF
Abstract

Spleen cells from mice immunized with sheep red cells were separated by differential adherence to insolubilized histamine, catecholamines, and prostaglandins. The hormones were insolubilized by linking them to Sepharose beads through a protein carrier. We measured hemolytic plaque formation (per million splenic leukocytes) of cells which passed through columns of hormone-carrier-Sepharose beads (i.e., those cells that failed to bind). As compared with control (no column) cells, the number of plaque-forming cells was substantially reduced by passage through histamine, epinephrine, isoproterenol, and prostaglandin-E2 columns. Plaque-forming cells were not significantly reduced by passage through carrier Sepharose (another control) or norepinephrine- and prostaglandin-F2α-carrier Sepharose columns. Thus, the ability of an insolubilized hormone preparation to subtract plaque-forming cells roughly correlated with the presence of pharmacologic receptors for the corresponding free hormones, as judged by stimulation of cyclic AMP accumulation in the same cells, reported previously. Both 19S and 7S plaque-forming cells were subtracted by columns prepared from pharmacologically active hormones, but none of the insolubilized hormones stimulated accumulation of intracellular cyclic AMP. The cell membrane phenomenon that allows adherence to a given hormone-carrier-bead column may be identical with the cell receptor.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 22
page 22
icon of scanned page 23
page 23
icon of scanned page 24
page 24
icon of scanned page 25
page 25
icon of scanned page 26
page 26
icon of scanned page 27
page 27
icon of scanned page 28
page 28
icon of scanned page 29
page 29
icon of scanned page 30
page 30
Version history
  • Version 1 (January 1, 1974): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts