Advertisement

Research Article Free access | 10.1172/JCI107469

Antihemophilic Factor Antigen. LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPY

Leon W. Hoyer, Rene P. de los Santos, and John R. Hoyer

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut 06032

Department of Pediatrics, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455

Find articles by Hoyer, L. in: JCI | PubMed | Google Scholar

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut 06032

Department of Pediatrics, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455

Find articles by de los Santos, R. in: JCI | PubMed | Google Scholar

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut 06032

Department of Pediatrics, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455

Find articles by Hoyer, J. in: JCI | PubMed | Google Scholar

Published November 1, 1973 - More info

Published in Volume 52, Issue 11 on November 1, 1973
J Clin Invest. 1973;52(11):2737–2744. https://doi.org/10.1172/JCI107469.
© 1973 The American Society for Clinical Investigation
Published November 1, 1973 - Version history
View PDF
Abstract

The tissue localization of antihemophilic factor (AHF, Factor VIII) has been determined by immunofluorescent studies using monospecific rabbit antibody to human AHF. Specific staining demonstrating AHF antigens has been identified in endothelial cells of a wide range of human tissues. The staining pattern was observed in endothelial cells of arteries, capillaries, and veins as well as the cells lining hepatic and splenic sinusoids. Specific fluorescence was limited to these endothelial cells in sections of kidney, liver, spleen, lymph node, cardiac and smooth muscle, thyroid, umbilical cord, and skin. Absorption studies established that the staining was specific for cells in which there were proteins that had AHF antigens. The demonstration of fluorescence within the cytoplasm of endothelial cells suggests that these cells synthesize proteins that have AHF antigens.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (November 1, 1973): No description
Advertisement
Advertisement