In vivo formation of H<sub>2</sub>O<sub>2</sub> in red cells during exposure to hyperoxia

William P. Johnson; Devenia Jefferson; Charles E. Mengel

doi:10.1172/JCI107029

In vivo formation of H₂O₂ in red cells during exposure to hyperoxia

Published August 1, 1972 - More info

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Abstract

Chow-fed and tocopherol-deficient mice were given aminotriazole (AT), exposed to 100% O₂ at 60 pounds per square inch absolute for 1 hr (OHP), and red blood cells were assayed for catalase activity and lipid peroxide levels. A decrease of catalase activity (CA) in the presence of AT can be taken as evidence of excess formation or accumulation of H₂O₂. No differences of CA were observed among chow-fed mice, with or without AT and/or OHP. Tocopherol-deficient mice with AT had lower CA (0.174±0.040) than chow-fed mice with AT (0.225±0.028) P < 0.01. Tocopherol-deficient mice with AT exposed to OHP had even lower CA, 0.137±0.024, P < 0.01.

The data are consistent with the hypothesis that H₂O₂ is formed or accumulated in excess in red cells of tocopherol-deficient mice, an effect that is enhanced in the presence of hyperoxia. They imply that tocopherol plays a role in the detoxification of H₂O₂.

In vivo formation of H₂O₂ in red cells during exposure to hyperoxia

William P. Johnson, Devenia Jefferson, and Charles E. Mengel

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In vivo formation of H2O2 in red cells during exposure to hyperoxia

William P. Johnson, Devenia Jefferson, and Charles E. Mengel

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In vivo formation of H₂O₂ in red cells during exposure to hyperoxia