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Free access | 10.1172/JCI106704

Metabolic alterations in the human erythrocyte produced by increases in glucose concentration: The role of the polyol pathway

Susan F. Travis, Anthony D. Morrison, Rex S. Clements Jr., Albert I. Winegrad, and Frank A. Oski

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

The Children's Hospital of Philadelphia, and the George S. Cox Institute, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

Find articles by Travis, S. in: JCI | PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

The Children's Hospital of Philadelphia, and the George S. Cox Institute, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

Find articles by Morrison, A. in: JCI | PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

The Children's Hospital of Philadelphia, and the George S. Cox Institute, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

Find articles by Clements, R. in: JCI | PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

The Children's Hospital of Philadelphia, and the George S. Cox Institute, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

Find articles by Winegrad, A. in: JCI | PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

The Children's Hospital of Philadelphia, and the George S. Cox Institute, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

Find articles by Oski, F. in: JCI | PubMed | Google Scholar

Published October 1, 1971 - More info

Published in Volume 50, Issue 10 on October 1, 1971
J Clin Invest. 1971;50(10):2104–2112. https://doi.org/10.1172/JCI106704.
© 1971 The American Society for Clinical Investigation
Published October 1, 1971 - Version history
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Abstract

Human erythrocytes incubated in medium containing 50 mM glucose have increased intracellular sorbitol and fructose concentrations as compared with samples incubated with 5 mM glucose. Increased medium glucose concentration did not significantly alter total glucose consumption or lactate production. However, the intracellular lactate:pyruvate ratio rose, the concentrations of fructose diphosphate, and triose phosphates increased, and the 2,3-diphosphoglycerate concentration fell. [14C]O2 production from glucose-1-14C also increased with increased medium glucose concentration. These changes are believed to reflect changes in the redox states of the diphosphopyridine nucleotide/reduced form of diphosphopyridine nucleotide (NAD/NADH) and nicotinamide—adenine dinucleotide phosphate/reduced form of nicotinamide—adenine dinucleotide phosphate (NADP/NADPH) couples resulting from increased activity of the polyol pathway. Addition of pyruvate to the incubation media prevented these changes. These studies illustrate that an increase in the red cell's normal substrate, glucose, can produce changes in red cell metabolism.

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