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Research Article Free access | 10.1172/JCI106419

Mechanism of action of thyroid hormones on erythrocyte 2,3-diphosphoglyceric acid synthesis

L. Michael Snyder and William J. Reddy

Department of Hematology, Saint Vincent Hospital, Worcester, Massachusetts 01604

Department of Biochemistry, Saint Vincent Hospital, Worcester, Massachusetts 01604

Find articles by Snyder, L. in: JCI | PubMed | Google Scholar

Department of Hematology, Saint Vincent Hospital, Worcester, Massachusetts 01604

Department of Biochemistry, Saint Vincent Hospital, Worcester, Massachusetts 01604

Find articles by Reddy, W. in: JCI | PubMed | Google Scholar

Published November 1, 1970 - More info

Published in Volume 49, Issue 11 on November 1, 1970
J Clin Invest. 1970;49(11):1993–1998. https://doi.org/10.1172/JCI106419.
© 1970 The American Society for Clinical Investigation
Published November 1, 1970 - Version history
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Abstract

Normal erythrocytes, when incubated with thyroid hormone, were found to have increased levels of 2,3-diphosphoglyceric acid. In addition, a partially purified enzyme preparation, when incubated with either a 1,3-diphosphoglyceric acid generating system or 1,3-diphosphoglyceric acid directly, showed increased levels of 2,3-diphosphoglyceric acid when exposed to thyroid hormone. The hormonal effect was biphasic and was witnessed after 5 min of incubation. Substitution on the 3 and 5 positions of the basic thyronine molecule was essential for hormonal effect. It appears that thyroid hormone acts by directly stimulating the diphosphoglycerate mutase enzyme. The hormonal effect on 2,3-DPG synthesis may offer a biochemical explanation for the shift in the oxyhemoglobin dissociation curve observed in thyroid disorders.

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