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Research Article Free access | 10.1172/JCI106281
Department of Pediatrics, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Find articles by Baehner, R. in: JCI | PubMed | Google Scholar
Department of Pediatrics, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Find articles by Gilman, N. in: JCI | PubMed | Google Scholar
Department of Pediatrics, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, and the Division of Research Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115
Find articles by Karnovsky, M. in: JCI | PubMed | Google Scholar
Published April 1, 1970 - More info
A comparison has been made of the metabolic shifts in human and guinea pig leukocytes when they phagocytize. Respiration of guinea pig polymorphonuclear leukocytes (PMN) and the increment during phagocytosis were each about 2½-fold that of human PMN. This was also true of the direct oxidation of glucose-6-P (hexose monophosphate shunt). Enzymes potentially responsible for these phenomena have been compared in each species. Cyanide-insensitive NADH oxidase and NADPH oxidase were measured and only the formed exhibited adequate activity to account for the respiratory stimulus durintg phagocytosis. The hydrogen peroxide formed by this enzyme stimulates the hexose monophosphate shunt by oxidizing glutathione which upon reduction by an NADPH-linked glutathione reductase provides NADP to drive the hexose monophosphate shunt. Other linkages between respiratory stimulation and that of the hexose monophosphate shunt also pertain in the guinea pig.