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Research Article Free access | 10.1172/JCI105937

Radioimmunoassay for human follicle—stimulating hormone: physiological studies

W. D. Odell, A. F. Parlow, C. M. Cargille, and G. T. Ross

Department of Medicine, Harbor General Hospital, Torrance, California 90509

Department of Obsterics and Gynecology, Harbor General Hospital, Torrance, California 90509

UCLA School of Medicine, Los Angeles, California 90024

Endocrinology Branch, National Cancer Institute, Bethesda, Maryland 20014

Find articles by Odell, W. in: JCI | PubMed | Google Scholar

Department of Medicine, Harbor General Hospital, Torrance, California 90509

Department of Obsterics and Gynecology, Harbor General Hospital, Torrance, California 90509

UCLA School of Medicine, Los Angeles, California 90024

Endocrinology Branch, National Cancer Institute, Bethesda, Maryland 20014

Find articles by Parlow, A. in: JCI | PubMed | Google Scholar

Department of Medicine, Harbor General Hospital, Torrance, California 90509

Department of Obsterics and Gynecology, Harbor General Hospital, Torrance, California 90509

UCLA School of Medicine, Los Angeles, California 90024

Endocrinology Branch, National Cancer Institute, Bethesda, Maryland 20014

Find articles by Cargille, C. in: JCI | PubMed | Google Scholar

Department of Medicine, Harbor General Hospital, Torrance, California 90509

Department of Obsterics and Gynecology, Harbor General Hospital, Torrance, California 90509

UCLA School of Medicine, Los Angeles, California 90024

Endocrinology Branch, National Cancer Institute, Bethesda, Maryland 20014

Find articles by Ross, G. in: JCI | PubMed | Google Scholar

Published December 1, 1968 - More info

Published in Volume 47, Issue 12 on December 1, 1968
J Clin Invest. 1968;47(12):2551–2562. https://doi.org/10.1172/JCI105937.
© 1968 The American Society for Clinical Investigation
Published December 1, 1968 - Version history
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Abstract

Most of the information concerning secretion changes in follicle-stimulating hormone (FSH) in humans has been gained with relatively insensitive bioassays of concentrates of pools of urine. We have developed a sensitive and specific radioimmunoassay for FSH that is 500-1000 times more sensitive than the rat ovarianweight augmentation assay and which is capable of quantifying FSH in small volumes of serum. Anti-FSH was prepared by immunizing rabbits with an impure FSH preparation. The majority of antisera showed complete inability to distinguish LH, TSH, and FSH, illustrating the immunological similarities of these hormones. One antiserum was specific when used in a radioimmunoassay. Potency estimates by bioassay were in good agreement, with a single exception, with those obtained with the radioimmunoassay for 10 FSH-containing preparations. Highly purified LH gave a higher potency by immunoassay than by bioassay.

Sera from eugonadal men contained 5-25 mIU/ml; sera from castrate men contained over 30 mIU/ml. Sera from eugonadal women contained 7-25 mIU/ml during the follicular phase and 5-15 mIU/ml during the luteal phase of the menstrual cycle. Sera from castrate or postmenopausal women contained 40-250 mIU/ml. FSH was measured throughout the menstrual cycle in 19 women. The general pattern that emerged is summarized as follows: there is a small early follicular phase rise in FSH, and then FSH is relatively constant until mid-cycle; in the majority of women a mid-cycle rise of FSH occurs coincidentally to the mid-cycle LH ovulatory peak; during the luteal phase FSH levels are relatively constant and lower than during the follicular phase. Nonsequential oral contraceptives containing estrogen and progestogen abolish these changes and FSH concentrations remain low throughout treatment. Treatment of castrate men and castrate or postmenopausal women with high doses of oral estrogens results in a fall of FSH to levels found in eugonadal men or women, but not to undetectable levels. Children less than 5 yr of age had undetectable FSH (< 5 mIU/ml).

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