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Research Article Free access | 10.1172/JCI105808

Suppression of glycine-15N incorporation into urinary uric acid by adenine-8-13C in normal and gouty subjects

J. Edwin Seegmiller, James R. Klinenberg, John Miller, and R. W. E. Watts

1Section on Human Biochemical Genetics, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

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1Section on Human Biochemical Genetics, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Klinenberg, J. in: PubMed | Google Scholar

1Section on Human Biochemical Genetics, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

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1Section on Human Biochemical Genetics, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

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Published May 1, 1968 - More info

Published in Volume 47, Issue 5 on May 1, 1968
J Clin Invest. 1968;47(5):1193–1203. https://doi.org/10.1172/JCI105808.
© 1968 The American Society for Clinical Investigation
Published May 1, 1968 - Version history
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Abstract

Adenine inhibited the de novo synthesis of purines in both normal and gouty man as shown by inhibition of the incorporation of glycine-15N into urinary uric acid without altering the incorporation of glycine-15N into urinary creatinine. The diminished purine synthesis did not result in a diminution in the 24 hr excretion of uric acid. This observation was explainable in part by the prompt conversion of adenine to uric acid. In addition to this direct conversion, adenine-8-13C provided a slow and prolonged contribution to urinary uric acid.

A feedback inhibition of purine synthesis by nucleotides derived from adenine provides the best interpretation of these results.

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