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Research Article Free access | 10.1172/JCI105697

Stimulation of Aldosterone Biosynthesis in Adrenal Mitochondria by Sodium Depletion

Elisa T. Marusic and Patrick J. Mulrow

Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut

‡

Postdoctoral Fellow USPHS, Grant I-FO 5 TW-883.

§

Research Career Development Award 5-K3-AM-4903.

Address requests for reprints to Dr. Patrick J. Mulrow, Yale University School of Medicine, 333 Cedar Street, New Haven, Conn. 06510.

*

Received for publication 7 July 1967 and in revised form 14 August 1967.

Presented in part at the Annual Meeting of the American Society for Clinical Investigation, 1 May 1967.

Supported by U. S. Public Health Service Research Grant AM 05954-06.

Find articles by Marusic, E. in: PubMed | Google Scholar

Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut

‡

Postdoctoral Fellow USPHS, Grant I-FO 5 TW-883.

§

Research Career Development Award 5-K3-AM-4903.

Address requests for reprints to Dr. Patrick J. Mulrow, Yale University School of Medicine, 333 Cedar Street, New Haven, Conn. 06510.

*

Received for publication 7 July 1967 and in revised form 14 August 1967.

Presented in part at the Annual Meeting of the American Society for Clinical Investigation, 1 May 1967.

Supported by U. S. Public Health Service Research Grant AM 05954-06.

Find articles by Mulrow, P. in: PubMed | Google Scholar

Published December 1, 1967 - More info

Published in Volume 46, Issue 12 on December 1, 1967
J Clin Invest. 1967;46(12):2101–2108. https://doi.org/10.1172/JCI105697.
© 1967 The American Society for Clinical Investigation
Published December 1, 1967 - Version history
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Abstract

The effect of various factors on the conversion of corticosterone to aldosterone was studied in an isolated mitochondrial system from rat adrenal glands. The adrenal mitochondrial fraction from rats on a low sodium diet has a greater capacity for converting corticosterone to aldosterone than mitochondria from rats fed a normal diet. After 1 day on a low sodium diet the amount converted was 162% and after the 2nd and 4th day the amounts converted were 239 and 242%, respectively, compared to a value of 100% for the control rats. Sodium and(or) potassium added in vitro did not affect the conversion of corticosterone to aldosterone.

The specificity of the sodium depletion stimulus on the conversion of corticosterone to aldosterone was established by comparing two other mitochondrial enzymes from glomerulosa cell mitochondria. Succinic dehydrogenase and 11 β-hydroxylase were measured in normal and sodium-depleted rats and no difference in activity of either enzyme was found.

The data are consistent with the view that sodium depletion stimulates the last step in aldosterone biosynthesis by causing a specific enzymatic change in adrenal mitochondria.

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