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Research Article Free access | 10.1172/JCI105694

Use of 125I- and 51Cr-Labeled Albumin for the Measurement of Gastrointestinal and Total Albumin Catabolism

Robert M. Kerr, John J. Du Bois, and Peter R. Holt

Gastrointestinal Research Laboratory, Department of Medicine, St. Luke's Hospital Center, New York

‡

Dr. Kerr's present address is the Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, N. C. 27103.

Address requests for reprints to Dr. Peter R. Holt, St. Luke's Hospital Center, New York 10025.

*

Received for publication 2 September 1966 and in revised form 24 July 1967.

A part of this work was presented at the annual meeting of the Federation of American Societies for Experimental Biology 1965, and published as an abstract (1).

Supported in part by grants from the New York Heart Association, the Health Research Council of New York (U-1292), and the U. S. Public Health Service (AM-8107 and HE-10055).

Find articles by Kerr, R. in: JCI | PubMed | Google Scholar

Gastrointestinal Research Laboratory, Department of Medicine, St. Luke's Hospital Center, New York

‡

Dr. Kerr's present address is the Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, N. C. 27103.

Address requests for reprints to Dr. Peter R. Holt, St. Luke's Hospital Center, New York 10025.

*

Received for publication 2 September 1966 and in revised form 24 July 1967.

A part of this work was presented at the annual meeting of the Federation of American Societies for Experimental Biology 1965, and published as an abstract (1).

Supported in part by grants from the New York Heart Association, the Health Research Council of New York (U-1292), and the U. S. Public Health Service (AM-8107 and HE-10055).

Find articles by Bois, J. in: JCI | PubMed | Google Scholar

Gastrointestinal Research Laboratory, Department of Medicine, St. Luke's Hospital Center, New York

‡

Dr. Kerr's present address is the Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, N. C. 27103.

Address requests for reprints to Dr. Peter R. Holt, St. Luke's Hospital Center, New York 10025.

*

Received for publication 2 September 1966 and in revised form 24 July 1967.

A part of this work was presented at the annual meeting of the Federation of American Societies for Experimental Biology 1965, and published as an abstract (1).

Supported in part by grants from the New York Heart Association, the Health Research Council of New York (U-1292), and the U. S. Public Health Service (AM-8107 and HE-10055).

Find articles by Holt, P. in: JCI | PubMed | Google Scholar

Published December 1, 1967 - More info

Published in Volume 46, Issue 12 on December 1, 1967
J Clin Invest. 1967;46(12):2064–2082. https://doi.org/10.1172/JCI105694.
© 1967 The American Society for Clinical Investigation
Published December 1, 1967 - Version history
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Abstract

A method for the simultaneous measurement of gastrointestinal protein loss and total albumin turnover entailing the use of a combination of 125iodine- and 51chromium-labeled albumin is described. Albumin turnover was calculated by the measurement of albumin-125I plasma decay and cumulative urinary excretion, and the results obtained agreed closely with previous studies utilizing albumin-131I. Gastrointestinal catabolism was calculated from the rate of fecal excretion of 51Cr and the specific activity of plasma albumin-51Cr, and these data were related to the calculated albumin turnover results. During the period of 6-14 days after administration, the ratio of specific activties of albumin-125I and -51Cr in plasma and in extravascular spaces or gastric and biliary secretions remained almost identical. Fecal excretion of 51Cr was also quite stable at this time. In six normal subjects gastrointestinal catabolism accounted for less than 10% of total albumin catabolism. Excessive gastrointestinal protein losses did not contribute to the low serum albumin in three patients with cirrhosis or in two adults with the nephrotic syndrome. Multiple mechanisms leading to hypoalbuminemia were demonstrated in other subjects with a variety of gastrointestinal disorders.

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