Immunoregulation of a viral model of multiple sclerosis using the synthetic cannabinoid R(+)WIN55,212
J. Ludovic Croxford, Stephen D. Miller
J. Ludovic Croxford, Stephen D. Miller
Published April 15, 2003
Citation Information: J Clin Invest. 2003;111(8):1231-1240. https://doi.org/10.1172/JCI17652.
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Article Autoimmunity

Immunoregulation of a viral model of multiple sclerosis using the synthetic cannabinoid R(+)WIN55,212

Abstract

Theiler murine encephalomyelitis virus–induced demyelinating disease (TMEV-IDD) is a mouse model of chronic-progressive multiple sclerosis (MS) characterized by Th1-mediated CNS demyelination and spastic hindlimb paralysis. Existing MS therapies reduce relapse rates in 30% of relapsing-remitting MS patients, but are ineffective in chronic-progressive disease, and their effects on disability progression are unclear. Experimental studies demonstrate cannabinoids are useful for symptomatic treatment of spasticity and tremor in chronic-relapsing experimental autoimmune encephalomyelitis. Cannabinoids, however, have reported immunosuppressive properties. We show that the cannabinoid receptor agonist, R(+)WIN55,212, ameliorates progression of clinical disease symptoms in mice with preexisting TMEV-IDD. Amelioration of clinical disease is associated with downregulation of both virus and myelin epitope-specific Th1 effector functions (delayed-type hypersensitivity and IFN-γ production) and the inhibition of CNS mRNA expression coding for the proinflammatory cytokines, TNF-α, IL1-β, and IL-6. Clinical trials investigating the therapeutic potential of cannabinoids for the symptomatic treatment of MS are ongoing, and this study demonstrates that they may also have potent immunoregulatory properties.

Authors

J. Ludovic Croxford, Stephen D. Miller

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Effects of cannabinoid receptor agonism on T cell proliferation depends ...
Effects of cannabinoid receptor agonism on T cell proliferation depends upon the cellular activation state. Early R(+)WIN55,212 treatment (filled circles) (postinfection day 0–5) significantly inhibited the proliferation of T lymphocytes compared with S(–)WIN55,212 (open circles) and untreated TMEV-infected mice (filled triangles) upon rechallenge with the TMEV VP270-86 peptide (postinfection day 8) (a), but not PLP139-151 (d). When measured at postinfection day 40, R(+)WIN55,212 treatment did not inhibit T lymphocyte proliferation to either VP270-86 or PLP139-151 peptides during established disease (postinfection day 26–31 or 50–55) (b, c, e, and f). *P < 0.05, compared with either S(–)WIN55,212 or untreated mice.