Lipid mediators from exudates were extracted using C18 solid-phase extraction and profiled using LC-MS/MS–based lipid mediator profiling. (A) MS/MS spectra employed for identification of lipid mediators. (B) Partial least squares discriminant analysis of exudate lipid mediator profiles: left panel, 2D score plot; right panel, corresponding 2D loading plot. (C–E) Cumulative levels for the lipid mediator from the (C) docosahexaenoic acid, (D) eicosapentaenoic acid, and (E) arachidonic acid SPMs, arachidonic acid–derived LTB4, and ratio of SPM to LTB4. Results shown are mean ± SEM of n = 11 females and n = 13 males for B–E. Statistical significance determined using Student’s 2-tailed unpaired t test; *P < 0.05 for C–E.