Pulmonary IC inflammation in mice receiving rhC5a. The induction of the inflammatory response in the lung was performed by intratracheal application of 150 μg of purified anti-OVA Ab, followed by systemic 20 mg/kg OVA Ag in C57BL/6 wild-type mice (IC) or WT mice treated with rhC5a (IC + rhC5a). Mice receiving only Ab or rhC5a served as controls (Ab, rhC5a). After 4 hours, lungs were lavaged and BALFs were assayed for PMN infiltration (upper left), pulmonary hemorrhage (upper right), and production of MIP-2 and TNF-α (lower panels). Data are expressed as means ± SEM (n = 6–13 mice for each group). Differences in IC as compared with ICs + rhC5a treatment groups were significant for all parameters (*P < 0.05, **P < 0.001).