Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood
Jacqueline D. Trudeau, … , Pere Santamaria, Rusung Tan
Jacqueline D. Trudeau, … , Pere Santamaria, Rusung Tan
Published January 15, 2003
Citation Information: J Clin Invest. 2003;111(2):217-223. https://doi.org/10.1172/JCI16409.
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Article Autoimmunity

Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood

Abstract

Autoimmune (type 1) diabetes mellitus results from the destruction of insulin-producing pancreatic β cells by T lymphocytes. Prediction of cell-mediated autoimmune diseases by direct detection of autoreactive T cells in peripheral blood has proved elusive, in part because of their low frequency and reduced avidity for peptide MHC ligands.

Authors

Jacqueline D. Trudeau, Carolyn Kelly-Smith, C. Bruce Verchere, John F. Elliott, Jan P. Dutz, Diane T. Finegood, Pere Santamaria, Rusung Tan

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NRP-V7–reactive T cells detected in peripheral blood indicate their pres...
NRP-V7–reactive T cells detected in peripheral blood indicate their presence in pancreatic islets. The mean (± SEM) percentage of NRP-V7 tetramer-positive CD8+ B220– cells within peripheral blood, pancreatic islets, spleen, and pancreatic draining lymph nodes are shown for individual NOD mice of 9–10 (gray bars; n = 7), 11–14 (black bars; n = 13), and 15–17 (white bars; n = 10) weeks of age. The inset shows a representative staining of each tissue from a single 12-week-old mouse. Numbers indicate the percentage of CD8+ B220– tetramer-positive cells.