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Research Article Free access | 10.1172/JCI1472

Gene transfer of CD40-ligand induces autologous immune recognition of chronic lymphocytic leukemia B cells.

K Kato, M J Cantwell, S Sharma, and T J Kipps

Division of Hematology/Oncology, Department of Medicine and the UCSD Human Gene Therapy Program, UCSD School of Medicine, La Jolla, California 92093-0663, USA.

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Division of Hematology/Oncology, Department of Medicine and the UCSD Human Gene Therapy Program, UCSD School of Medicine, La Jolla, California 92093-0663, USA.

Find articles by Cantwell, M. in: JCI | PubMed | Google Scholar

Division of Hematology/Oncology, Department of Medicine and the UCSD Human Gene Therapy Program, UCSD School of Medicine, La Jolla, California 92093-0663, USA.

Find articles by Sharma, S. in: JCI | PubMed | Google Scholar

Division of Hematology/Oncology, Department of Medicine and the UCSD Human Gene Therapy Program, UCSD School of Medicine, La Jolla, California 92093-0663, USA.

Find articles by Kipps, T. in: JCI | PubMed | Google Scholar

Published March 1, 1998 - More info

Published in Volume 101, Issue 5 on March 1, 1998
J Clin Invest. 1998;101(5):1133–1141. https://doi.org/10.1172/JCI1472.
© 1998 The American Society for Clinical Investigation
Published March 1, 1998 - Version history
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Abstract

CD40-CD40-ligand (CD154) interactions play a critical role in immune activation. Using replication defective adenovirus encoding mouse CD154 (Ad-CD154), we modified human chronic lymphocytic leukemia B cells to express a functional ligand for CD40. This not only induces expression of immune accessory molecules on the infected cell, but also allows it to trans-activate noninfected bystander leukemia B cells. Also, factors that impair the antigen-presenting capacity of leukemia B cells are downmodulated. Ad-CD154- infected leukemia cells are highly effective stimulators in mixed lymphocyte reactions and can induce generation of cytotoxic T lymphocytes specific for autologous nonmodified leukemia cells. As such, Ad-CD154 can induce a host antileukemia response that may have therapeutic potential.

Version history
  • Version 1 (March 1, 1998): No description
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