Advertisement

Research Article Free access | 10.1172/JCI119340

In utero exposure to helminth and mycobacterial antigens generates cytokine responses similar to that observed in adults.

I Malhotra, J Ouma, A Wamachi, J Kioko, P Mungai, A Omollo, L Elson, D Koech, J W Kazura, and C L King

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Malhotra, I. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Ouma, J. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Wamachi, A. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Kioko, J. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Mungai, P. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Omollo, A. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Elson, L. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Koech, D. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by Kazura, J. in: JCI | PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4983, USA.

Find articles by King, C. in: JCI | PubMed | Google Scholar

Published April 1, 1997 - More info

Published in Volume 99, Issue 7 on April 1, 1997
J Clin Invest. 1997;99(7):1759–1766. https://doi.org/10.1172/JCI119340.
© 1997 The American Society for Clinical Investigation
Published April 1, 1997 - Version history
View PDF
Abstract

Neonates exposed to parasite antigens (Ags) in utero may develop altered fetal immunity that could affect subsequent responses to infection. We hypothesized that cord blood lymphocytes (CBL) from offspring of mothers residing in an area highly endemic for schistosomiasis, filariasis, and tuberculosis in Kenya would either fail to respond or generate a predominantly Th2-associated cytokine response to helminth and mycobacterial antigens (PPD) in vitro compared to maternal PBMC. Kenyan CBL generated helminth Ag-specific IL-5 (range 29-194 pg/ml), IL-10 (121-2,115 pg/ml), and/or IFN-gamma (78 pg/ml-10.6 ng/ml) in 26, 46, and 57% of neonates, respectively (n = 40). PPD induced IFN-gamma in 30% of Kenyan CBL (range 79-1,896 pg/ml), but little or no IL-4 or IL-5. No Ag-specific IL-4, IL-5, or IFN-gamma release was detected by CBL obtained in the United States (n = 11). Ag-driven cytokine production was primarily CD4-dependent. Cytokine responses to helminth and mycobacterial Ags by maternal PBMC mirrored that observed in neonates. CBL from helminth infected and/or PPD-sensitized mothers produced more Ag-specific cytokines compared to CBL from uninfected mothers (P < 0.05). These data demonstrate that the human fetus develops similar patterns of cytokine production observed in adults and indicates that prenatal exposure may not lead to tolerance or altered fetal immunity. .

Version history
  • Version 1 (April 1, 1997): No description
Advertisement
Advertisement