Expression of suppressors of cytokine signaling during liver regeneration
Jean S. Campbell, … , Peter C. Heinrich, Nelson Fausto
Jean S. Campbell, … , Peter C. Heinrich, Nelson Fausto
Published May 15, 2001
Citation Information: J Clin Invest. 2001;107(10):1285-1292. https://doi.org/10.1172/JCI11867.
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Article

Expression of suppressors of cytokine signaling during liver regeneration

Abstract

The cytokines TNF and IL-6 play a critical role early in liver regeneration following partial hepatectomy (PH). Since IL-6 activates signal transducers and activators of transcription (STATs), we examined whether the suppressors of cytokine signaling (SOCS) may be involved in terminating IL-6 signaling. We show here that SOCS-3 mRNA is induced 40-fold 2 hours after surgery. SOCS-2 and CIS mRNA are only weakly induced, and SOCS-1 is not detectable. SOCS-3 induction after PH is transient and correlates with a decrease in STAT-3 DNA binding and a loss of tyrosine 705 phosphorylation. This response is markedly reduced in IL-6 knockout (KO) mice. TNF injection induces SOCS-3 mRNA in wild-type mice (albeit weakly compared with the increase observed after PH) but not in TNF receptor 1 or IL-6 KO mice. In contrast, IL-6 injection induces SOCS-3 in these animals, demonstrating a requirement for IL-6 in SOCS-3 induction. IL-6 injection into wild-type mice also induces SOCS-1, -2, and CIS mRNA, in addition to SOCS-3. Together, these results suggest that SOCS-3 may be a key component in downregulating STAT-3 signaling after PH and that SOCS-3 mRNA levels in the regenerating liver are regulated by IL-6.

Authors

Jean S. Campbell, Lisa Prichard, Fred Schaper, Jochen Schmitz, Alyssa Stephenson-Famy, Maryland E. Rosenfeld, Gretchen M. Argast, Peter C. Heinrich, Nelson Fausto

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Figure 5

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TNF injection induces SOCS-3 and CIS mRNA in a time-dependent manner in ...
TNF injection induces SOCS-3 and CIS mRNA in a time-dependent manner in WT, but not in TNF-R1 KO and IL-6 KO mouse liver. Total RNA was prepared from tissue obtained from the median liver lobe at the indicated times after TNF injection (25 μg/kg, intraperitoneally). (a) Total RNA from WT mice was probed for SOCS-3, CIS, and cyclophilin message levels as described in Methods. Molecular-weight markers are shown on the left in kilobases. I and II represent RNA prepared from duplicate mice. (b) Induction of SOCS-3 and CIS mRNA levels was quantified by phosphorimager analysis as described in Figure 2. Quantification of SOCS-3 and CIS level is shown in black and gray bars, respectively, for WT, TNF-R1–, and IL-6 KO mice. Similar time-course experiments were done on TNF-R1– and IL-6 KO mice, and total RNA was prepared and probed as described in a for WT mice. The error bars represent SEM of the data from three independent experiments. AP < 0.001 vs. control values.