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Research Article Free access | 10.1172/JCI115365

Targeted and highly efficient gene transfer into CD4+ cells by a recombinant human immunodeficiency virus retroviral vector.

T Shimada, H Fujii, H Mitsuya, and A W Nienhuis

Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

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Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

Find articles by Fujii, H. in: JCI | PubMed | Google Scholar

Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

Find articles by Mitsuya, H. in: JCI | PubMed | Google Scholar

Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

Find articles by Nienhuis, A. in: JCI | PubMed | Google Scholar

Published September 1, 1991 - More info

Published in Volume 88, Issue 3 on September 1, 1991
J Clin Invest. 1991;88(3):1043–1047. https://doi.org/10.1172/JCI115365.
© 1991 The American Society for Clinical Investigation
Published September 1, 1991 - Version history
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Abstract

We have established a recombinant HIV gene transfer system based on transient expression of the HIV packaging functions and a recombinant vector genome in monkey kidney Cos cells. The recombinant HIV retroviral vector introduced the neoR gene into CD4+ cells with high efficiency, comparable to that achieved with the highest titer amphotropic murine recombinant retrovirus. Vector preparations were devoid of replication competent, infectious HIV. Gene transfer was dependent on CD4 expression, as shown by expression of the CD4 gene in HeLa cells, and could be inhibited by soluble CD4. This specific and efficient gene transfer system may be useful for development of gene therapy for which T cells are the desired targets.

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