Usage Information

A highly informative probe for two polymorphic Vh gene regions that contain one or more autoantibody-associated Vh genes.
P P Chen, … , R A Erger, D A Carson
P P Chen, … , R A Erger, D A Carson
Published August 1, 1989
Citation Information: J Clin Invest. 1989;84(2):706-710. https://doi.org/10.1172/JCI114218.
View: Text | PDF
Research Article

A highly informative probe for two polymorphic Vh gene regions that contain one or more autoantibody-associated Vh genes.

Abstract

Efforts to determine the role of specific Ig variable region (V) genes in human autoimmune responses have been hampered by the lack of suitably polymorphic probes. Recently we isolated a heavy chain V (Vh) gene, designated Humhv3005, that is 99% homologous to the 1.9III Vh gene and can encode an anti-DNA antibody. To study the relation between these two genes, different DNA fragments from the isolated Humhv3005 clone were used to probe Southern blots of human genomic DNA. A 1.6-kb Eco RI fragment (designated hv3005/E1.6) was found to hybridize with only one band in Eco RI-digested DNA, and with two major bands in Bam HI-digested DNA. Importantly, the sizes of the latter two bands were indistinguishable from the corresponding Bam HI fragment sizes of the isolated hv3005 clone and the isolated 1.9III clone, respectively. Population and family studies with the hv3005/E1.6 probe revealed five different hybridization patterns of these two characteristic bands, which defined nine possible genotypes for two human Ig Vh gene loci. Together the data demonstrate that hv3005/E1.6 is a highly informative probe for an autoantibody-associated Vh gene(s), and should prove useful in elucidating the role of Ig Vh genes in autoimmune diseases.

Authors

P P Chen, K A Siminovitch, N J Olsen, R A Erger, D A Carson

×

Usage data is cumulative from February 2024 through February 2025.

Usage JCI PMC
Text version 73 0
PDF 48 15
Figure 0 1
Scanned page 134 2
Citation downloads 43 0
Totals 298 18
Total Views 316
(Click and drag on plot area to zoom in. Click legend items above to toggle)

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.

Advertisement