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Research Article Free access | 10.1172/JCI114158

Expression of diverse Na+ channel messenger RNAs in rat myocardium. Evidence for a cardiac-specific Na+ channel.

M N Sills, Y C Xu, E Baracchini, R H Goodman, S S Cooperman, G Mandel, and K R Chien

Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Department of Medicine, University of California, San Diego, La Jolla 92093.

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Published July 1, 1989 - More info

Published in Volume 84, Issue 1 on July 1, 1989
J Clin Invest. 1989;84(1):331–336. https://doi.org/10.1172/JCI114158.
© 1989 The American Society for Clinical Investigation
Published July 1, 1989 - Version history
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Abstract

This study examined the diversity of Na+ channel gene expression in intact cardiac tissue and purified myocardial cells. The screening of neonatal rat myocardial cell cDNA libraries with a conserved rat brain Na+ channel cDNA probe, resulted in the isolation and characterization of a putative rat cardiac Na+ channel cDNA probe (pCSC-1). The deduced amino acid sequence of pCSC-1 displayed a striking degree of homology with the eel, rat brain-1, and rat brain-2 Na+ channel, thereby identifying pCSC-1 as a related member of the family of Na+ channel genes. Northern blot analysis revealed the expression of a 7-kb CSC-1 transcript in rat cardiac tissue and purified myocardial cells, but little or no detectable expression of CSC-1 in rat brain, skeletal muscle, denervated skeletal muscle, or liver. Using RNase protection and Northern blot hybridization with specific rat brain Na+ channel gene probes, expression of the rat brain-1 Na+ channel was observed in rat myocardium, but no detectable expression of the rat brain-2 gene was found. This study provides evidence for the expression of diverse Na+ channel mRNAs in rat myocardium and presents the initial characterization of a new, related member of the family of Na+ channel genes, which appears to be expressed in a cardiac-specific manner.

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