Localization of the gene for coagulation factor XIII a-chain to chromosome 6 and identification of sites of synthesis.

L J Weisberg; D T Shiu; C S Greenberg; Y W Kan; M A Shuman

doi:10.1172/JCI112862

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Published in Volume 79, Issue 2 on February 1, 1987
J Clin Invest. 1987;79(2):649–652. https://doi.org/10.1172/JCI112862.
© 1987 The American Society for Clinical Investigation

Published February 1, 1987 - Version history

Factor XIII, the clotting factor essential for covalent stabilization of the fibrin clot, is a heterodimer consisting of a2 and b2 subunits, with catalytic function residing in the a-chain. In order to address questions regarding sites of synthesis and chromosomal localization of the Factor XIII a-chain, cDNA was cloned from a lambda gt11 human placental cDNA library. Nucleotide and amino acid sequences were determined from the cDNA. Amino acid sequencing of purified platelet Factor XIII a-chains confirmed the authenticity of the lambda gt11 clone. The gene for Factor XIII a-chain was mapped uniquely to chromosome 6. Northern blot analysis of human placental and U937 (monocytelike) cell poly (A)+ mRNA showed a single approximately 4.0-kb message for the Factor XIII a-chain. These results provide conclusive evidence that the a-chain is synthesized by placenta and monocyte cell lines.

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