Advertisement

Research Article Free access | 10.1172/JCI108917

Immunologic and Nonimmunologic Generation of Superoxide from Mast Cells and Basophils

William R. Henderson and Michael Kaliner

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases National Institutes of Health, Bethesda, Maryland 20014

Find articles by Henderson, W. in: JCI | PubMed | Google Scholar

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases National Institutes of Health, Bethesda, Maryland 20014

Find articles by Kaliner, M. in: JCI | PubMed | Google Scholar

Published January 1, 1978 - More info

Published in Volume 61, Issue 1 on January 1, 1978
J Clin Invest. 1978;61(1):187–196. https://doi.org/10.1172/JCI108917.
© 1978 The American Society for Clinical Investigation
Published January 1, 1978 - Version history
View PDF
Abstract

Mediator release from rat peritoneal and human lung mast cells as well as human leukemic basophils was examined to determine whether super-oxide (O−2) was concomitantly generated. Immunologic or nonimmunologic stimulation of each preparation induced parallel release of histamine and O−2 within 2 min. O−2 production was quantitated by superoxide dismutase (SOD)-inhibitable chemiluminescence and cytochrome c reduction. SOD was detected in basophil and mast cell lysates and was also released by rat mast cells stimulated by anti-IgE. Secretory granules isolated from purified rat mast cells released histamine, O−2, and SOD upon exposure to cations. Thus, both superoxide radicals and SOD may play a role in host defenses involved in immediate hypersensitivity reactions.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (January 1, 1978): No description
Advertisement
Advertisement