Wendy M. Macfarlane, Timothy M. Frayling, Sian Ellard, Julie C. Evans, Lisa I.S. Allen, Michael P. Bulman, Susan Ayres, Maggie Shepherd, Penny Clark, Ann Millward, Andrew Demaine, Terence Wilkin, Kevin Docherty, Andrew T. Hattersley
Localization of IPF-1 mutants. Western blot analysis of cytoplasmic (lanes
1-5) or nuclear (lanes 6-10) samples prepared from Nes2y cells which were
incubated in low (3 mM) or high (20 mM) glucose concentrations. Lanes 1 and
6 represent untransfected Nes2y cells, lanes 2 and 7 cells expressing normal
IPF-1, lanes 3 and 8 cells expressing C18R, lanes 4 and 9 cells expressing
D76N, and lanes 5 and 10 cells expressinfg R197H. Analysis was preformed
using a specific IPF-1 antibody, with 1 μg of each extract being used.
Results are representative of three separate experiments.