CCK-8 induced inhibition of food intake is mediated through the CCK-A
receptor. After an overnight fast, animals were injected with either saline
or CCK-8 and provided access to chocolate-flavored Ensure as described in
Methods. Cumulative intake (mean ± SEM) over a 15-min period
after injection is shown. Significance vs. intake after saline injection (0
μg CCK-8/kg body weight [BW]):
*P < 0.05,
**P < 0.01. (a) CCK-8 induced, dose-dependent inhibition of food intake is observed
in wild-type (WT), but not in CCK-AR–/–
mice. Food consumption by 10 wild-type and 10
CCK-AR–/– animals was compared. ANOVA
parameters were [F(3,39) = 89.23, P
< 0.0001] and [F(3,39 )=
0.16, P = 0.92] for comparisons
among wild-type and CCK-AR–/– animals,
respectively. (b). CCK-8 induced, dose-dependent inhibition of food intake is
observed in both wild-type and CCK-BR–/–
mice. Food consumption by 9 wild-type and 10
CCK-BR–/– animals was compared. ANOVA
parameters were [F(3,35) = 44.40, P
< 0.0001] and [F(3,39) =
135.21, P < 0.0001] for comparisons
among wild-type and CCK-BR–/– animals,
respectively. BW, body weight.