(A) Fluorescence changes in response to 10 μM SubP
and 10 μM carbachol. Cell diameters in images are approximately
20 microns. (B) [Ca2+]i versus time for
10 cells from images in A, measured in response to sequential
pulses of 10 μM SubP and 10 μM carbachol.
(C) [Ca2+]i versus time for 10 cells
from images in A, measured in response to sequential pulses of
10 μM SubP without and with 5 μM atropine (Atr).
Fluorescence ratio, 340 nm/380 nm. (D) Mean response to SubP in
presence or absence of BAPTA-AM (500 μM); 4 experiments from 2
HN and 1 DC subjects (16–20 glands). Error bars are SEM.
(E) Mean response to SubP in the absence and presence of
clotrimazole (25 μM), which blocks Ca2+-activated
K+ channels (n = 4, 27–42
glands). *P < 0.05 versus SubP responses. Error bars
are SEM.