Niels C. Riedemann, Ren-Feng Guo, Thomas A. Neff, Ines J. Laudes, Katie A. Keller, Vidya J. Sarma, Maciej M. Markiewski, Dimitrios Mastellos, Christoph W. Strey, Carl L. Pierson, John D. Lambris, Firas S. Zetoune, Peter A. Ward
Inhibition of binding of 125I-labeled mouse C5a (125I-C5a) to peritoneal mouse neutrophils in vitro (a) and various organs in vivo (b) by αC5aR. (a) Binding of 125I-C5a is expressed as cpm. Groups were treated (as indicated) for 20 minutes prior to binding of 125I-C5a. Data are representative of five independent experiments per condition. (b) Binding of 125I-C5a is expressed as the ratio of cpm per g organ to cpm in 100 μl blood from each animal (n = 4 per condition) obtained 10 minutes after intravenous injection of 125I-C5a and 6 hours after CLP. αC5aR (6 μg/mouse) or an equal amount of irrelevant rabbit IgG (control) was administered intravenously 15 minutes prior to 125I-C5a infusion. *Statistical significance in treated groups when compared with control animals. Ctrl, control.