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Concise Publication Free access | 10.1172/JCI107393
1Department of Medicine, Division of Nephrology, Albert Einstein College of Medicine, Bronx, New York 10461
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1Department of Medicine, Division of Nephrology, Albert Einstein College of Medicine, Bronx, New York 10461
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1Department of Medicine, Division of Nephrology, Albert Einstein College of Medicine, Bronx, New York 10461
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Published August 1, 1973 - More info
The permeability of the toad bladder to a series of isotopically labeled nonelectrolytes was determined in the presence of 150 mM unlabeled acetamide. Under these conditions, overall bladder function was unimpaired, as shown by a normal response to vasopressin of short-circuit current and permeability coefficient of [3H]water,[14C]ethanol, and [14C]propionamide. The permeability of the bladder to isotopic acetamide and urea, however, was significantly depressed by unlabeled acetamide, in both the absence and presence of vasopressin. These experiments indicate a competition between unlabeled and isotopic species for binding sites, and show the existence of a saturable, vasopressin-sensitive carrier for urea and acetamide in the epithelial cell membrane.
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