(A) Representative image of cycled and continuous semaglutide-treated (SEMA-treated) mice on day 120. (B) Body weight trajectories of Cohort 1 cycled (red, n = 6) and continuous (black, n = 7) mice. GTT, glucose tolerance testing; ITT, insulin tolerance testing. (C) Body weights of individual cycled mice 14 days after each cycle. (D) Fat, lean, and total mass by EchoMRI on day 83. (E) Mean adipocyte area in eWAT and iWAT, quantified from representative H&E-stained sections (F) (scale bar, 100 μm). (G) Plasma leptin levels in Cohort 1. (H) Body weight trajectories of Cohort 2 cycled (red, n = 6) and continuous (black, n = 7) mice over 95 days; green star, 1 missed treatment day. (I) Fat, lean, and total mass by EchoMRI on day 95. (J) Percentage weight change in cycled versus continuous mice across both cohorts. Unpaired 2-tailed Student’s t test. NS, not significant. *P < 0.05, **P < 0.01. Data presented as mean ± SEM.