Abstract
Authors
Caitlin M. Pavelec, Leigh A. Bradley, Priyanka Rawat, Luke S. Dunaway, Maya Bolger-Chen, Bethany A. Gholson, Jonathan R. Lindner, Brant E. Isakson, Norbert Leitinger, Matthew J. Wolf
Figure 1
I.V. Iron induces cardiac ferroptosis in murine cardiometabolic heart failure.
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(A ) Experimental protocol for mice fed control chow or HFHS diet and given a dose of IV iron. (B ) Volcano plot of genes expressed in hearts of mice fed HFHS or chow and give iron (n = 2–3 samples per group). Solid line indicates a –log10(P adj) values of 1.3. Genes highlighted in green are significantly changed and included in the ferroptosis pathway (WikiPathways). (C ) Heatmap of the expression of ferroptosis genes (WikiPathways) from hearts of mice fed control chow or HFHS that were given i.v. iron. Scale is –2 to +2, normalized for counts per million. (D and E ) Confocal microscopy and quantification of oxC11-BODIPY staining of hearts of mice fed control chow or HFHS diet given IV. n = 3 control group and n = 5 HFHS group. Scale bars: 10 μm. P value by 2-tailed Student t test. (F ) Quantification of total oxidized phospholipids by LC-MS/MS comparing hearts of HFHS diet that were given vehicle (n = 4) or i.v. iron (n = 5). P value by Student’s t test.
