Intravesical pressure and afferent discharge were recorded simultaneously during continuous filling from an ex vivo preparation. (A) Diagram of the nerve recording chamber and recording setup. (B and C) Representative recordings of intravesical pressure, raw nerve activity, and afferent discharge from a bladder of a naive control (B) and an SK2-KO mouse (C). Bladder infusion initiation is denoted by a red arrow. (D) Apparent bladder capacity in SK2-KO and WT littermate mice. Urinary bladders were infused at a rate of 15 μL/min until the intravesical pressure reached 40 cmH₂O. Apparent bladder capacity represents the volume required to increase the intravesical pressure to 40 cmH₂O. (E) Mean afferent nerve discharge frequency at 40 cmH₂O for SK2-KO and WT littermate mice. (F) Normalized afferent discharge as a function of intravesical pressure for bladders of WT and SK2-KO mice. Data are shown as the mean ± SEM (WT, n = 6; SK2-KO, n = 7; 2-way ANOVA, *P < 0.05). (G) Bladder capacity as a function of intravesical pressure for bladders of WT and SK2-KO mice. No difference in bladder capacity versus pressure was observed between WT and SK2-KO.