Advertisement
Research Article Free access | 10.1172/JCI106305
Division of Hematology of the Department of Medicine, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115
Find articles by Baehner, R. in: JCI | PubMed | Google Scholar
Division of Hematology of the Department of Medicine, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115
Find articles by Nathan, D. in: JCI | PubMed | Google Scholar
Division of Hematology of the Department of Medicine, Children's Hospital Medical Center, Boston, Massachusetts 02115
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115
Find articles by Karnovsky, M. in: JCI | PubMed | Google Scholar
Published May 1, 1970 - More info
Polymorphonuclear leukocytes from patients with chronic granulomatous disease (CGD) exhibit metabolic and bactericidal deficiencies that may be the result of inadequate production of H2O2. A hydrogen peroxide-generating system was, therefore, inserted into CGD leukocytes. This was accomplished by allowing the cells to phagocytize latex spherules coated with glucose oxidase. This produced an amelioration in the known metabolic deficiencies of these cells during phagocytosis: (a) intracellular (catalatic) formate oxidation dependent upon hydrogen peroxide production was enhanced fourfold; and (b) hexose monophosphate shunt activity, which other workers have shown to be at least partially dependent upon the availability of H2O2, was markedly stimulated. These data strengthen the evidence that the fundamental metabolic lesion in CGD cells during phagocytosis is indeed deficient production of hydrogen peroxide, probably, as previously shown, due to diminished oxidase for reduced nicotinamide adenine dinucleotide.
Images.