The hypoxia-inducible factor α pathway couples angiogenesis to osteogenesis during skeletal development
J. Clin. Invest. Ying Wang, et al. 117:1616 doi:10.1172/JCI31581 [Go to this article.]

Figure 8
Bone and vascular phenotype in Vhl and Hif1a double-knockout mice. (A) Representative μCT images of femoral cross sections from 6-week-old ΔVhl/ΔHif1a double mutant mice and wild-type controls. Scale bars: 1.0 mm. (B) Representative images of Microfil-perfused femurs from 3-week-old ΔVhl/ΔHif1a double-mutant mice and control littermates. Scale bar: 1.0 mm. (C) Immunohistochemical analysis of HIF-2α level in femoral sections from 6-week-old ΔVhl/ΔHif1a double-mutant mice and control littermates. Sections were counterstained with hematoxylin. Red arrows indicate positive and black arrows negative staining in osteoblasts. Original magnification, ×400. (D) In situ hybridization analysis with Vegf mRNA on histological sections from 6-week-old control and double-mutant femurs. Original magnification, ×100.