Targeted gene deletion in Candida parapsilosis demonstrates the role of secreted lipase in virulence
J. Clin. Invest. Attila Gácser, et al. 117:3049 doi:10.1172/JCI32294 [
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Figure 2Lipase activity of
C. parapsilosis WT and lipase mutants.
(
A) Effect of
C. parapsilosis WT and lipase mutants on rhodamine B–FCS agar plate. Lipolytic activities are demonstrated by the red-stained regions around the colonies. 1, WT strain; 2, homozygous mutant; 3, heterozygous mutant; and 4, reconstituted mutant. (
B) Rhodamine B–FCS agar plate irradiated with UV light. Fluorescent halos indicate lipolytic activity. The experiment was performed 3 times with similar results. (
C) Relative extracellular lipolytic activity of
C. parapsilosis WT, heterozygous
CpLIP1-2/Δcplip1-2 (HE), homozygous
Δcplip1-2/ Δcplip1-2 (KO), and reconstituted
Δcplip1-2/CpLIP2 (RE) mutants. Lipolytic activities of supernatants of overnight cultures in YNB–olive oil medium were measured using paranitrophenyl palmitate. Error bars indicate SD. *
P < 0.0001 (ANOVA).
