Blocking aggrecanase cleavage in the aggrecan interglobular domain abrogates cartilage erosion and promotes cartilage repair
J. Clin. Invest. Christopher B. Little, et al. 117:1627 doi:10.1172/JCI30765 [Go to this article.]

Figure 1
Mutation detection by Southern blotting and PCR. (A) Southern blotting of genomic DNA detected 9.3- and 4.9-kb BamHI fragments in wild-type and Jaffa alleles, respectively. (B) After stripping, a 1.6-kb fragment was detected in Jaffa DNA on the same filter probed with a neo cDNA probe. (C) The mutation that changed amino acids ³⁷⁴ALG to ³⁷⁴NVY introduced a unique AflIII restriction site in exon 7. (D) A 758-bp PCR fragment amplified from wild-type DNA was resistant to AflIII digestion. AflIII digestion of the PCR fragment amplified from Jaffa (J) and heterozygote (Het) DNA yielded 623-bp and 135-bp fragments, although the 135-bp fragment was rarely visible on the gels.