Interplay of IKK/NF-κB signaling in macrophages and myofibers promotes muscle degeneration in Duchenne muscular dystrophy
J. Clin. Invest. Swarnali Acharyya, et al. 117:889 doi:10.1172/JCI30556 [
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Figure 3Heterozygous deletion of p65 rescues
mdx pathology.
(
A) Histopathology of muscle cryosections stained with H&E, comparing 5-week-old
mdx;
p65+/+ and
mdx;p65+/– mice or
mdx;p50+/+ and
mdx;p50+/– mice. Scale bars: 20 μm. (
B) Gastrocnemius sections from WT C57BL/10,
mdx;p65+/+, and
mdx;
p65+/– mice were immunostained with F4/80. Scale bar: 50 μm. (
C) RNA was isolated from the remaining part of the muscle, and real-time PCR was performed for lysozyme and CD68, comparing 5-week-old WT C57BL/10,
mdx;p65+/+, and
mdx;p65+/– mice (
n = 3). (
D) Necrotic fibers from gastrocnemius muscles were quantitated based on IgG staining (filled fibers appear in red, nuclei in blue) (
n = 5). Scale bar: 100 μm. (
E) Calcifications were quantitated from
mdx;p65+/+ and
mdx;
p65+/– muscles (
n = 10). Data are plotted as mean ± SEM from 2 independent experiments. *
P < 0.05.
