Familial Alzheimer disease–linked mutations specifically disrupt Ca²⁺ leak function of presenilin 1
J. Clin. Invest. Omar Nelson, et al. 117:1230 doi:10.1172/JCI30447 [Go to this article.]

Figure 3
Summary of PS1-FAD rescue experiments. (A) The average basal cytosolic Ca²⁺ levels (gray bars) and the amplitude of BK-induced Ca²⁺ responses (black bars) are shown as mean ± SD for DKO cells transfected with EGFP and PS1 expression constructs (the number of cells analyzed is indicated above each set of bars). When compared with DKO cells transfected with EGFP alone, the basal Ca²⁺ levels were significantly higher and the amplitude of BK-induced Ca²⁺ responses was significantly smaller in DKO cells transfected with EGFP+PS1 and EGFP+PS1-A79V combinations. [Ca²⁺]_i, intracellular Ca²⁺ concentration. (B) The average size of IO-releasable Ca²⁺ pool is shown as mean ± SD for DKO cells transfected with EGFP and PS1 expression constructs (the number of cells analyzed is shown above each bar). When compared with DKO cells transfected with EGFP alone, the size of IO-releasable Ca²⁺ pool was significantly smaller in DKO cells transfected with EGFP+PS1 and EGFP+PS1-A79V combinations. (C) The average [Ca²⁺]_ER level is shown as mean ± SD for DKO cells transfected with EGFP and PS1 expression constructs (the number of cells analyzed is shown above each bar). When compared with DKO cells transfected with EGFP alone, the [Ca²⁺]_ER levels were significantly smaller in DKO cells transfected with EGFP+PS1 and EGFP+PS1-A79V combinations. ***P < 0.05 by ANOVA.