Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis
J. Clin. Invest. Stephanie Humblet-Baron, et al. 117:407 doi:10.1172/JCI29539 [Go to this article.]

Figure 5
WASp^–/– Tregs exhibit in vitro suppressive activity. CD4⁺CD25^– effector T cells (T_EFF) and CD4⁺CD25⁺ cells (Tregs) were isolated from WT or WASp^–/– mice (C57BL/6 strain). WT or WASp^–/– T_EFF were labeled with CSFE and plated as targets with WT or WASp^–/– Tregs at the Treg/T_EFF (target) ratios noted in the presence of irradiated APCs. Cultures were stimulated with 3 μg/ml anti-CD3 and 1 μg/ml anti-CD28 for 110 hours. Relative CFSE dilution was measured in cultures containing WT (A) or WASp^–/– (B) targets. Unstimulated and control-stimulated (CD3/CD28 without Tregs) cells are shown in the left panels.