CD4⁺ T cell–independent DNA vaccination against opportunistic infections
J. Clin. Invest. Mingquan Zheng, et al. 115:3536 doi:10.1172/JCI26306 [Go to this article.]

Figure 7
Functionality and antigen specificity of serum after pKexin/CD40L vaccination in mice. (A) Opsonic activity of serum after DNA immunization with control, pKexin, or pKexin/CD40L vectors. (B) Passive transfer of serum or CD19⁺ splenocytes confers protection against a PC challenge in SCID mice. n = 4–6. All data are mean values ± SEM. *P < 0.05, **P < 0.01 compared with control. (C) Representative Western blot analysis of serum after Kexin vaccination against sonicated PC antigen. Neither naive serum nor serum from CD4-depleted mice with active PC infection gave any activity on Western blotting. Serum from mice undergoing 3 rounds of pKexin/CD40L vaccination (far right 2 lanes) reacted with a 105- and 55-kDa protein. (D) Representative immunofluorescent staining of mouse and monkey PC with anti-Kexin serum.