CD4⁺ T cell–independent DNA vaccination against opportunistic infections
J. Clin. Invest. Mingquan Zheng, et al. 115:3536 doi:10.1172/JCI26306 [Go to this article.]

Figure 4
Proteomics of PC antigens. (A) Immunoprecipitation of PC antigens after DC-based vaccination in mice with 1 antigen identified at 55 kDa. N-terminal sequencing and tandem MS analysis of this antigen showed greater than 85% homology with Kexin. M denotes molecular weight marker. (B) Typhoon images of 2D gels of Cy5-labeled naive serum (left) or immunoprecipitated PC antigens (right). Proteins were separated within pH 5–8 for the first dimension. Boxes indicate proteins that were analyzed by matrix-assisted laser desorption/ionization (MALDI) TOF/TOF mass spectrometry.