Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [Go to this article.]

Figure 3
Histology of intestinal villi in uninjected WT (left), WT injected with iron (center), and weh^Tp85c–/– zebrafish injected with iron (right). Perls’ stain performed at 6 months of age (A) and 12 months of age (B) revealed nonheme iron accumulation in the intestinal villi of weh^Tp85c–/– zebrafish but not in controls. Magnification, ×40. Scale bar: 100 microns. (C) DAB-enhanced Perls’ staining for nonheme iron at 12 months for the same specimens as shown in B.