Defining the pathogenic involvement of desmoglein 4 in pemphigus and staphylococcal scalded skin syndrome
J. Clin. Invest. Takeshi Nagasaka, et al. 114:1484 doi:10.1172/JCI20480 [Go to this article.]

Figure 5
Immunoadsorption and purification of Dsg4-reactive IgG autoantibodies from PF sera. Two of the PF sera (A and B) were applied to a column that contained hDsg4-His, and the Dsg4 reactivities of the sera were examined by IP-IB analysis both before and after column purification. We injected the IgG fractions, which were prepared from the sera with or without immunoadsorption by hDsg4-His, into neonatal mice to examine their pathogenic activities (see Figure 7). IgG that was affinity-purified on the hDsg4-His column was eluted (eluted fraction) and examined for its reactivities against Dsg1, Dsg3, and Dsg4. The IgG affinity-purified on Dsg4 cross-reacted with Dsg1 but not with Dsg3.