Distinct roles of Smad pathways and p38 pathways in cartilage-specific gene expression in synovial fibroblasts
J. Clin. Invest. Hiroaki Seto, et al. 113:718 doi:10.1172/JCI19899 [
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Figure 7Histological analysis of knee joints in the mouse ACL and MM resection model. (A–F) Toluidine blue staining (A and B) and type X collagen immunostaining (C and D) at the marginal area between the articular cartilage and synovium. B and D present higher-magnification views of A and C, respectively. Osteochondrophytes were formed at the posterior edge of the femoral condyle, and they were positively stained by anti–type X collagen as well as toluidine blue (rectangular areas in A and C). Clusters of migrating synovial cells were observed adjacent to the osteochondrophytes (B, arrowheads) where future osteochondrophytes will develop, and they were positively stained by anti–type X collagen at the marginal area between synovium and osteophytes (rectangular area in D). This region was also positively stained by anti–phospho-p38 (F). E and F represent phase-contrast microscopy (E) and immunostaining with anti–phospho-p38 (F) of the rectangular area in D. Positive phospho-p38 staining was observed at the area of osteochondrophytes as well as the marginal synovium. Scale bars: 500 μm (A–D) and 50 μm (E and F).
