Stat-3 is required for pulmonary homeostasis during hyperoxia
J. Clin. Invest. Isamu Hokuto, et al. 113:28 doi:10.1172/JCI19491 [
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Figure 8Decreased surfactant proteins in
Stat-3Δ/Δ mice following hyperoxia. (
a) SP-A, SP-B, SP-C, and SP-D were quantitated in BAL fluid by Western blot analysis. In room air, SP-A, SP-B, SP-C, and SP-D were not altered (lanes 3 and 4). After exposure to 95% O
2 for 65 hours, SP-A and SP-B were significantly decreased in the
Stat-3Δ/Δ mice (lanes 7–9),
P < 0.01, after quantitative imaging. (
b) SP-A, SP-B, and SP-C mRNAs were quantitated by S1 nuclease assay. In room air, SP-A, SP-B, and SP-C mRNAs were similar in
Stat-3Δ/Δ and control mice. After exposure to 95% O
2 for 65 hours, SP-A, SP-B, and SP-C mRNAs were decreased in
Stat-3Δ/Δ compared with control mice. Results were standardized to L32 mRNA and normalized to room air controls,
n = 5 per group. Statistical differences were assessed by Student’s
t test, *
P < 0.01 versus control O
2.
