Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia
J. Clin. Invest. Guang Yang, et al. 114:669 doi:10.1172/JCI19300 [
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Figure 6Bcl-2 expression and TUNEL staining in lungs of WT mice exposed to 72 hours of hyperoxia. (
A) Bcl-2 mRNA levels were evaluated by RT-PCR. Densitometric values are expressed as a ratio to GAPDH. Values are the mean ± SE of 3 separate experiments. *
P < 0.05 vs. air-exposed mice.
† P < 0.05 vs similarly exposed neonates. (
B) Immunoreactive Bcl-2 and Bax protein levels. (
C) Immunohistochemical staining of Bcl-2 protein. Note that increased Bcl-2 was localized predominantly in the alveolar epithelial cells (arrowhead). (
D) TUNEL staining in mouse lung tissues after hyperoxia. Note the relatively low number of TUNEL-positive cells in the neonatal lung but the high signal intensity in the similarly exposed adult lung (yellow).
