Inactivation of Icmt inhibits transformation by oncogenic K-Ras and B-Raf
J. Clin. Invest. Martin O. Bergo, et al. 113:539 doi:10.1172/JCI18829 [Go to this article.]

Figure 6
Growth factor-stimulated Erk1/2 and Akt1 phosphorylation in Icmt-deficient fibroblasts. (a) Nontransfected Icmt^flx/flx and the derivative Icmt^Δ/Δ fibroblasts were seeded at equal density and serum-starved overnight as described in the Methods section. Serum-containing medium was then added to the cells. Cells were harvested at the indicated time points and analyzed by immunoblotting with antibodies against phosphorylated Erk1/2 (p-Erk1/2), phosphorylated Akt1 (p-Akt1), and total Erk1/2. (b) Icmt^flx/flx and the derivative Icmt^Δ/Δ fibroblasts were seeded at equal density and serum-starved overnight. Medium (0.5% serum) supplemented with EGF (50 ng/ml) was added to the cells. Cells were harvested at the indicated time points and analyzed by immunoblotting with antibodies against p-Erk1/2 and total Erk1/2.